A brain metastasis model for breast cancer using human embryonic stem cell-derived cerebral organoids

Background: Breast cancer is a common cause of brain metastasis. Although breast has relatively high survival rates, its rate after metastasis to the lower. Conventional two-dimensional cell culture models and animal are widely used in metastatic research, these have tremendously contributed understanding this disease. However, some limitations, such as different physiological features genetic backgrounds.Methods: We established simple model using human pluripotent stem cell-derived cerebral organoids (COs)—in case, (BC-COs).Results: Using BC-CO model, we induced MDA-MB-231 cells into COs by co-culture with compared differences between adapted BC-COs non-adapted cells. Our results showed that proliferative capacity increased Additionally, expression levels endothelial-mesenchymal transition markers were significantly higher cells.Conclusion: conclude promotes also could be novel tool for research on cancer.